Botanisches Kolloquium im WS 16/17
am Institut für Allgemeine Botanik
Ankündigung und Einladung
Dr. Kyle J. Lauersen
CeBiTec, Universität Bielefeld
hält am Montag, 30.01.2017 um 1715 Uhr im SR 215 (Johann-Joachim-Becher-Weg 7, Erdgeschoss) folgenden Vortrag:
„Catching up with the times:
Advanced genetic engineering tools and
applications for the eukaryotic microalga Chlamydomonas reinhardtii“
Microalgae hold promise as hosts for sustainable bio-production processes. These photosynthetic organisms can be cultivated with freely available sunlight energy while sequestering atmospheric CO2 as a carbon source. Although microalgae are natural sources of many interesting bio-products, expanding the range and value of the compounds produced by these organisms through genetic engineering can increase the economic competitiveness of algal production platforms. Although significant advancements in genetic manipulation and understanding of transgene regulation in microalgae have been achieved, genetic manipulation of these organisms has lagged behind other biotechnologically relevant hosts such as bacteria and yeasts. Our work has focused on engineering nuclear transgene expression in the Chlorophyceae microalga Chlamydomonas reinhardtii, with the aim of generating heterologous bio-products from this photosynthetic host. We employ several levels of optimization in gene design, followed by synthetic development of nuclear cassettes in order to ensure robust expression of target constructs. Recently in our group, the development of a fully synthetic and optimized gene expression vector (pOptimized) further increased the range of possibilities for gene expression. We have applied this system for numerous tasks including: heterologous gene overexpression for recombinant protein production via secretion into culture media, in vivo localization studies of native proteins, and metabolic engineering for novel isoprenoid generation from C. reinhardtii. Our workflow allows reliable engineering, previously unattainable in this host, and serves as a model for future engineering work in other industrially relevant microalgal strains. This presentation will overview our recent successes in engineering nuclear transgene expression in C. reinhardtii for numerous applications.
Gäste sind herzlich willkommen!
Die Professoren der Allgemeinen Botanik