Publikationen / Publications

Horn, R. and Paulsen, H. (2002) Folding in vitro of light-harvesting chlorophyll a/b protein is coupled with pigment binding. J. Mol. Biol. 318:547-556.

Gerken, U.; Wolf-Klein, H; Huschenbett, C.; Götze, B.; Schuler, S.; Jelezko, F.; Tietz, C.; Wrachtrup, J., and Paulsen, H. (2002) Single molecule spectroscopy of oriented recombinant trimeric light harvesting complexes of higher plants. Single Mol. 3: 183-188.

Melkozernov, A.N.; Schmid, V.H.R.; Lin, S.; Paulsen, H. and Blankenship, R.E. (2002) Excitation energy transfer in the Lhca1 subunit of LHC I-730 peripheral antenna of photosystem I. J. Phys. Chem. B 106: 4313-4317.

Wolf-Klein, H.; Kohl, C.; Müllen, K., and Paulsen, H. (2002) Biomimetic model of a plant photosystem consisting of a recombinant light-harvesting complex and a terrylene dye. Angew. Chem. 114: 3526-3529; Angew. Chem. Int. Ed. 41: 3380-3382.

Phillip, D.; Hobe, S.; Paulsen, H.; Molnar, P.; Hashimoto, H., and Young, A.J. (2002) The binding of xanthophylls in the bulk light-harvesting complex of photosystem II of higher plants. A specific requirement for carotenoids with a 3-hydroxy ß-end group. J. Biol. Chem. 277: 25160-25169.

Schmid, V.H.R.; Paulsen, H. and Rupprecht, J. (2002) Identification of N- and C-terminal amino acids of Lhca1 and Lhca4 required for formation of the heterodimeric peripheral photosystem I antenna LHCI-730. Biochemistry 41: 9126-9131.

Schmid, V.H. R.; Potthast. S.; Wiener, M.; Bergauer, V.; Paulsen, H. and Storf, S. (2002) Pigment binding of photosystem I light-harvesting proteins. J. Biol. Chem. 277: 37307-37314.

Hobe, S.; Fey, H., Rogl, H., and Paulsen, H. (2003) Determination of relative chlorophyll binding affinities in the major light-harvesting chlorophyll-a/b complex. J. Biol. Chem. 278: 5912-5919.

Yang, C., Horn, R. & Paulsen, H. (2003) Light-harvesting chlorophyll a/b complexes (LHCIIb) can be reconstituted in vitro from its completely unfolded apoprotein. Biochemistry 42: 4527-4533

Schmidt, K.; Fufezan, C.; Krieger-Liszkay, A.K; Satoh, H. & Paulsen H. (2003) Recombinant water-soluble chlorophyll protein from Brassica oleracea var. botrys binds various chlorophyll derivatives. Biochemistry 42: 7427-7433.

Mick, V.; Eggert, K.; Heinemann, B.; Geister, S. & Paulsen, H. (2004) Single amino acids in the luminal loop domain influence the stability of the major light-harvesting chlorophyll a/b complex (LHCIIb). Biochemistry 43: 5467-5473.

Jeschke, G.; Bender, A.; Paulsen, H.; Zimmermann, H. & Godt, A. (2004) Sensitivity enhancement in pulse EPR distance measurements. J. Magn. Reson. 169: 1-12.

Jeschke, G.; Panek, G.; Godt, A.; Bender, A. & Paulsen, H. (2004) Data analysis procedure for pulse ELDOR measurements of broad distance distributions. Appl. Magn. Reson. 26: 223-244.

Rühle, W. & Paulsen, H. (2004) Preparation of native and recombinant light-harvesting chlorophyll-a/b complex. In: Photosynthesis Research Protocols (Carpentier, R., Ed.), Humana Press Inc., Totowa, NJ, S. 93-104.

Horn, R. & Paulsen, H. (2004) Early steps in the assembly of light-harvesting chlorophyll a/b complex. J. Biol. Chem. 279 (43), 44400-44406.

Mick, V.; Geister, S. & Paulsen, H. (2004) Folding state of the lumenal loop determines the thermal stability of light-harvesting chlorophyll a/b protein (LHCIIb). Biochemistry 43: 14704-14711.

Jeschke, G.; Bender, A.; Schweikardt, T.; Panek, G.; Decker, H. & Paulsen, H. (2005) Localization of the N-terminal domain in light-harvesting chlorophyll a/b protein (LHCIIb) by electron paramagnetic resonance (EPR) measurements. J. Biol. Chem. 280: 18623--18630.

Hobe, S.; Trostmann, I.; Raunser, S. & Paulsen, H. (2006) Assembly of the major light-harvesting chlorophyll-a/b complex. Thermodynamics and kinetics of neoxanthin binding. J. Biol. Chem.281: 25156-25166

Yang, C.; Boggasch, S.; Haase, W. & Paulsen, H. (2006) Thermal stability of trimeric light-harvesting chlorophyll a/b complex (LHCIIb) in liposomes of thylakoid lipids,. Biochim. Biophys. Acta – Bioenergetics 1757: 1642-1648.

Paulsen, H. (2006) Reconstitution and Pigment Exchange. In: Advances in Photosynthesis and Respiration, Vol.: Chlorophylls and Bacteriochlorophylls, Biochemistry, Biophysics, Functions, and Applications (Grimm, B.; Porra, R.; Rüdiger, W. & Scheer, H., Ed.), Kluwer Academic Publishers, Dordrecht, The Netherlands, S. 275-385.

Horn, R.; Grundmann, G. & Paulsen, H. (2007) Consecutive binding of chlorophylls a and b during the assembly in vitro of light-harvesting chlorophyll a/b protein (LHCIIb). J. Mol. Biol. 366: 1045-1054.

Renger, T.; Trostmann, I.; Theiss, C.; Madjet, M.E.; Richter, M.; Paulsen, H.; Eichler, H.J.; Knorr, A. & Renger, G. (2007) Refinement of a structural model of a pigment-protein complex by accurate optical line shape theory and experiments. J. Phys. Chem. B 111, 10487-10501.

Corbet, D.; Schweikardt, T.; Paulsen, H.; Decker, H. & Schmid, V.H.R. (2007) Amino acids in the second transmembrane helix of the Lhca4 subunit are important fort he formation of stable heterodimeric light-harvesting complex LHCI-730. J. Mol. Biol. 370, 170-182.

Theiss, C.; Trostmann, I.; Andree, S.; Schmitt, F.J.; Renger, T.; Eichler, H.J.; Paulsen, H. & Renger, G. (2007) Pigment-pigment and pigment-protein interactions in recombinant water-soluble chlorophyll proteins (WSCP) from cauliflower. J. Phys. Chem. B 111, 13325-13335.

Liu, J, Lauterbach, R.; Paulsen, H. & Knoll, W. (2008) Immobilization of light-harvesting chlorophyll a/b complex (LHCIIb) studied by surface plasmon field-enhanced fluorescence spectroscopy. Langmuir 24, 9661-9667.

Yang, C.; Lambrev, P.; Cheng, Z.; Jávorfi, T.; Kiss, A.Z.; Paulsen, H. & Garab, G. (2008) The negatively charged amino acids in the lumenal loop influence the pigment binding and the conformation of the major light-harvesting chlorophyll a/b complex of photosystem II. Biochim. Biophys. Acta Bioenerget. 1777, 1463-1470.

Frommolt, R.; Werner, S.; Paulsen, H.; Goss, R.; Wilhelm, C.; Zauner, S.; Maier, U.G.; Grossman, A.R.; Bhattacharya, D. & Lohr, M. (2008) Ancient recruitment by chromists of green algal genes encoding enzymes for carotenoid biosynthesis. Mol. Biol. Evol.25, 2653-2667.

Schmitt, F.J.; Trostmann, I.; Theiss, C.; Pieper, J.; Renger, T.; Fuesers, J.; Hubrich, E.H.; Paulsen, H.; Eichler, H.J. & Renger, G. (2008) Excited states dynamics in recombinant water-soluble chlorophyll proteins (WSCP) from cauliflower investigated by transient fluorescence spectroscopy. J. Phys. Chem. B 112, 13951-13961.

Volkov, A.; Dockter, C.; Bund, T.; Paulsen, H. & Jeschke, G. (2009) Pulse EPR determination of water accessibility to spin-labelled amino acid residues in LHCIIb. Biophys. J. 96, 1124-1141.

Zhou, F.; Liu, S.; Hu, Z.; Kuang, T.; Paulsen, H. & Yang, C. (2009) Effect of monogalactosyldiacylglycerol on the interaction between photosystem II core complex and its antenna complexes in liposomes of thylakoid lipids. Photosynth. Res. 99, 185-193.

Gundlach, K.; Werwie, M.; Wiegand, S. and Paulsen, H. (2009) Filling the “green gap” of the major light-harvesting chlorophyll a/b complex by covalent attachment of Rhodamine Red. Biochim. Biophys. Acta 1787, 1499-1504.

Renger, T.; Madjet, M.E.; Müh, F.; Trostmann, I.; Schmitt, F.J.; Theiss, C.; Paulsen, H.; Eichler, H.J.; Knorr, A., and Renger, G. (2009) Thermally activated superradiance and intersystem crossing in the water-soluble chlorophyll-binding protein. J. Phys. Chem. B 113, 9948-9957.

Dockter, C.; Volkov, A.; Bauer, C.; Polyhach Y.; Joly-Lopez, Z. Jeschke, G., and Paulsen, H. (2009) Refolding of the integral membrane protein light-harvesting complex II monitored by pulse EPR. Proc. Natl. Acad. Sci. USA 106, 18485-18490.

Erker, W.; Boggasch, S.; Xie, R.; Grundmann, G.; Paulsen, H. und Basché, T. (2010) Assemblies of semiconductor quantum dots and light-harvesting complex II. J. Lumin. 130, 1624-1627.

Volkov, A.; Dockter, C.; Polyhach, Y.; Paulsen, H., and Jeschke, G. (2010) Site-specific information on membrane protein folding by electron spin echo envelope modulation spectroscopy. J. Phys. Chem. Lett. 1, 663-667.

Paulsen, H.; Dockter, C.; Volkov, A. and Jeschke, G. (2010) Folding and pigment binding of light-harvesting chlorophyll a/b protein. In: The Chloroplast (Rebeiz, C.A.; Benning, C.; Bohnert, H.J.; Daniell, H.; Hoober, J.K.; Lichtenthaler, H.K.; Portis A.R. und Tripathy, B.C., Eds.) Advances in Photosynthesisi and Respiration Vol. 31, Springer, Dordrecht, The Netherlands, pp. 231-244.

Peneva, K.; Gundlach, K.; Herrmann, A.; Paulsen, H. and Müllen K. (2010) Site-specific incorporation of perylene into an N-terminally modified light-harvesting complex II. Org. Biomol. Chem. 8, 4823-4826.

Lauterbach, R.; Liu, J.; Knoll, W. and Paulsen, H. (2010) Energy transfer between surface-immobilized light-harvesting chlorophyll a/b complex (LHCII) studied by surface plasmon field –enhanced fluorescence spectroscopy. Langmuir 26, 17315-17321.

Kibrom, A.; Roskamp. R.F.; Jonas, U.; Menges, B.; Knoll, W.; Paulsen, H. and Naumann, R.L.C. (2011) Hydrogel-supported protein-tethered bilayer lipid membranes: a new approach toward polymer-supported lipid membranes. Soft Matter 7, 237-246.

Pieper, J.; Rätsep, M.; Trostmann, I.; Schmitt, F.; Theiss, C.; Eichler, C.; Paulsen, H.; Freiberg, A. and Renger, G. (2011a) Excitonic energy level structure and pigment-protein interactions in the recombinant water-soluble chlorophyll protein (WSCP). I. Difference fluorescence line narrowing. J. Phys. Chem. B 115, 4053-4065.

Pieper, J.; Rätsep, M.; Trostmann, I.; Schmitt, F.; Theiss, C.; Eichler, C.; Paulsen, H.; Freiberg, A. and Renger, G. (2011b) Excitonic energy level structure and pigment-protein interactions in the recombinant water-soluble chlorophyll protein (WSCP). II. Spectral hole-burning experiments. J. Phys. Chem. B 115, 4042-4052.

Renger, G.; Pieper, J.; Theiss, C.; Trostmann, I.; Paulsen, H.; Renger, T.; Eichler, H.J. and Schmitt, F.-J. (2011) Water-soluble chlorophyll binding protein of higher plants: A most suitable model system for basic analyses of pigment-pigment and pigment-protein interactions in chlorophyll-protein complexes. J. Plant Physiol. 108, 1462-1472.

Dockter, C.; Müller, A.H.; Dietz, C.; Volkov, A.; Polyhach, Y.; Jeschke, G. and Paulsen, H. (2012) Rigid core and flexible terminus: structure of solubilized light-harvesting chlorophyll a/b complex (LHCII) measured by EPR. J. Biol. Chem. 287, 2915-2925.

Bektas, I.; Fellenberg, C. and Paulsen, H. (2012) Water-soluble chlorophyll protein (WSCP) of Arabidopsis is expressed in the gynoecium and developing silique. Planta 236, 251-259.

Werwie, M.; Xu, X.; Haase, M.; Basché, T. and Paulsen, H. (2012) Bio serves nano: biological light-harvesting complex as energy donor for semiconductor quantum dots. Langmuir 28, 5810-5818.

Werwie, M.; Fehr, N.; Xu, X.; Basché, T. and Paulsen H. (2014) Comparison of quantum dot-binding protein tags: affinity determination by ultracentrifugation and FRET. Biochim. Biophys. Acta - Gen. Subjects 1840, 1651-1656.

Schüler, T.; Renkel, J.; Hobe, S.; Susewind, M.; Jacob, D.E.; Panthöfer, M.; Hoffmann-Röder, A.; Paulsen, H. and Tremel, W. (2014) Designed peptides for biomineral polymorph recognition: a case study for calcium carbonate. J. Mater. Chem. B 2, 3511-3518.

Roeder, S; Hobe, S. and Paulsen, H. (2014) Silica encapsulation for significantly stabilized, energy-conducting light-harvesting complex (LHCII). Langmuir 30, 14234-13240.

Basché, T.; Köhn, A.; Gauss, J.; Müllen, K.; Paulsen, H. and Zentel, R. (2014) From single molecules to nanoscopically structured materials: Optical properties of assemblies of molecules and nanoparticles. Adv. Polymer Sci. 260, 61-113.

Deserno, M.; Kremer, K.; Paulsen, H.; Peter, C. and Schmid F. (2014) From single molecules to nanoscopically structured materials: computational studies of biomembrane systems: theoretical considerations, simulation models, and applications. Adv. Polymer Sci. 260, 237-283.

Sun, R.; Liu, K.; Dong, L.; Wu, Y.; Paulsen, H. and Yang, C. (2015) Direct energy transfer from the major antenna to the photosystem II core complexes in the absence of minor antennae in liposomes. Biochim. Biophys. Acta 1847, 248-261.

Zerfaß, C.; Braukmann, S.; Nietzsche, S.; Hobe, S. and Paulsen, H. (2015) High yield recombiant production of a self-assembling polycationic peptide for silica biomineralization. Prot. Expr. Purif. 108, 1-8.

Rusevich, L.; Embs, J.; Bektas, I.; Paulsen, H.; Renger, G. and Pieper, J. (2015) Protein and solvent dynamics of the water-soluble chlorophyll-binding protein (WSCP). EPI Web of Conferences 83, 02016.1-02016.4.

Fehr, N.; Dietz, C.; Polyhach, Y.; von Hagens, T.; Jeschke, G. and Paulsen, H (2015) Modelling of the N-terminal section and the lumenal loop of trimeric light-harvesting complex II (LHCII) by using EPR. J. Biol. Chem. 290, 26007-26020.

Debnath, A.; Wiegand, S.; Paulsen, H.; Kremer, K. and Peter C. (2015) Derivation of coarse-grained simulation models of chlorophyll molecules in lipid bilayers for application in light-harvesting systems. Phys. Chem. Chem. Phys. 17, 22054-22063.

Zapf, T.; Tan, C.W.; Reinelt, T.; Huber, C.; Shaohua, D.; Geifman-Shochat, S.; Paulsen, H. and Sinner, E.K. (2015) Synthesis and functional reconstitution of light-harvesting complex II (LHCII) into polymeric membrane architectures. Angew. Chem. Int. Ed. 54, 4664-4668.

Fehr, N.; García-Rubio, I.; Jeschke, G. and Paulsen, H. (2016) Early folding events during light harvesting complex II assembly in vitro monitored by pulsed electron paramagnetic resonance. Biochim. Biophys. Acta 1857, 695-704.